Rabbit anti-NUP153 Antibody

SKU: orb1527787

Description

NUP153 Antibody

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 50 and 100
• Target: NUP153
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

HNUP153; nucleoporin 153kDa; nucleoporin Nup153; nucleoporin 153kD; N153; 153 kDa nucleoporin; nuclear pore complex protein Nup153; nuclear pore complex protein hnup153

Detection of human NUP153 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-NUP153 antibody orb1527787 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 second (B).

Detection of human NUP153 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 (Cat. No.orb1527787) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

NUP153

UniProt

P49790

RefSeq (Protein):NP_005115.2