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Catalog Number | orb1879430 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NUP153 |
Clonality | Polyclonal |
Tested applications | FC, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NUP153. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000), FC (1/10 - 1/50) |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Entrez | 9972 |
UniProt ID | P49790 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Alternative names | Nuclear pore complex protein Nup153; 153 kDa nucle Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of NUP153 expression in Hela (A) whole cell lysates. (Predicted band size: 153 kD; Observed band size: 140 kD)
Flow cytometric analysis of 293 cells using Anti-NUP153 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
ELISA, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
Human | |
Rabbit | |
Polyclonal | |
FITC |
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