Rabbit anti-Nucleolin (NCL) Antibody

SKU: orb1529755

Description

Nucleolin/NCL Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:1,000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 660 and C-terminus
• Target: Nucleolin/NCL

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

C23; Protein C23; Nsr1; nucleolin

Detection of human and mouse Nucleolin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Nucleolin used at a dilution of 1:1, 000.

Detection of human and mouse Nucleolin by WB (h&m) and IP (h).Samples: HeLa, HEK293T, mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Nucleolin antibody used for WB at 0.02 µg/ml (A), 1 µg/ml (B) and for IP at 3 µg/mg lysate (B).

Detection of mouse Nucleolin by IHC. Sample: mouse cerebellum. Antibody: Affinity purified rabbit anti-Nucleolin at 1:100. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.

Quick Database Links

Gene Symbol

Nucleolin/NCL

UniProt

P19338

RefSeq (Protein):NP_005372.2