Nucleolin/NCL Rabbit Polyclonal Antibody

SKU: orb570341

Description

The Nucleolin/NCL Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, IHC, and WB applications. This antibody targets the Nucleolin in Human, Monkey, Mouse, and Rat samples.

Research Area

Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Nucleolin/NCL recombinant protein (Position: K219-A629).
• Target: Nucleolin
• Molecular Weight: 100-110 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C23; NCL; nucleolin; Protein C23

Quick Database Links

Gene Symbol

Nucleolin

UniProt

P19338