Rabbit NEK4 Antibody

SKU: orb1526204

Description

NEK4 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 500 and 550
• Target: NEK4
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

never in mitosis A-related kinase 4; NIMA (never in mitosis gene a)-related kinase 4; nimA-related protein kinase 4; NRK2; pp12301; serine/threonine kinase 2; serine/threonine protein kinase-2; Serine/threonine-protein kinase 2; serine/threonine-protein kinase Nek4; serine/threonine-protein kinase Nek4 variant 2; serine/threonine-protein kinase NRK2; STK2

Detection of human NEK4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody orb1526204 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human NEK4 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NEK4 (Cat. No. orb1526204) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NEK4

UniProt

P51957

RefSeq (Protein):NP_003148.2