NEK4 antibody [N2C1], Internal

• Catalog Number: orb556056
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NEK4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NEK4. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 0.37 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 95
• Target: NEK4
• UniProt ID: P51957
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: NIMA related kinase 4 , NRK2 , STK2 , pp12301
• Note: For research use only
• Application notes: Positive control: A431 , H1299 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using NEK4 antibody

Immunohistochemical staining of A549 xenograft using NEK4 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using NEK4 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using NEK4 antibody