Rabbit anti-MED12 Antibody

• Catalog Number: orb1529662
• Category: Antibodies
• Description: MED12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 2127 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MED12
• UniProt ID: Q93074
• NCBI: NP_005111.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: OHDOX; trinucleotide repeat-containing gene 11 pro
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Med12 by western blot.Samples: Whole cell lysate from HeLa, A-549, GaMG, Hep-G2, and K-562 cells. Antibody: Affinity purified rabbit anti-Med12 antibody at 0.04 µg/ml.

Detection of mouse Med12 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-Med12 antibody at 0.04 µg/ml.

Detection of human MED12 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of mouse MED12 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Med12 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Med12 antibody.