Mouse Med12 Antibody (C-term)

• Catalog Number: orb1926372
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB52354
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 244561 Da
• Target: This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.
• UniProt ID: A2AGH6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Med12 Antibody (C-term) at 1:1000 dilution + mouse spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Med12 Antibody (C-term) at 1:1000 dilution + NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Med12 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: C2C12 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 245 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.