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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ICC-IF - 1:200 - 1:1000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 93 and C-terminus |
| Target | H2AX |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; histone H2AX
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Detection of human and mouse H2AX by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-H2AX antibody orb1530714 at 0.1 µg/ml.
Detection of mouse H2AX by immunohistochemistry.Sample:FFPE section of mouse renal cell carcinoma.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human H2AX by immunohistochemistry.Sample:FFPE section of human ovarian carcinoma.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human H2AX by IHC.Sample:FFPE section of HCT 116 cell line.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000. Detection:DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002096.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
Rabbit H2AX Antibody (orb1530714)
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