Rabbit GTF2E1/TFIIE-alpha Antibody

SKU: orb1526052

Description

GTF2E1/TFIIE-alpha Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-GTF2E1/TFIIE-alpha antibody A302-755A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 100 and 150
• Target: GTF2E1/TFIIE-alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FE; general transcription factor IIE 56 kDa subunit; general transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1, alpha 56kDa; TF2E1; TFIIE alpha subunit; TFIIE-A; TFIIE-alpha; transcription initiation factor IIE subunit alpha

Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody orb1526052 used for IP at 3 µg/mg lysate.

Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Cat. No. orb1526052) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

GTF2E1/TFIIE-alpha

UniProt

P29083

RefSeq (Protein):NP_005504.2