Rabbit GTF2E1/TFIIE-alpha Antibody

SKU: orb1526050

Description

GTF2E1/TFIIE-alpha Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 389 and C-terminus
• Target: GTF2E1/TFIIE-alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FE; general transcription factor IIE 56 kDa subunit; general transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1, alpha 56kDa; TF2E1; TFIIE alpha subunit; TFIIE-A; TFIIE-alpha; transcription initiation factor IIE subunit alpha

Detection of human and mouse GTF2E1/TFIIE-alpha by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody orb1526050 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human and mouse GTF2E1/TFIIE-alpha by immunohistochemistry.Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Cat. No. orb1526050) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

GTF2E1/TFIIE-alpha

UniProt

P29083

RefSeq (Protein):NP_005504.2