Rabbit GR Antibody

SKU: orb1524737

Description

GR Antibody

Research Area

Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Sheep
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 150 and 200
• Target: GR
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GCCR; GCR; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; GR; GRL; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Detection of human GR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GR antibody orb1524737 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. GR was also immunoprecipitated by rabbit anti-GR antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

Detection of human GR by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-GR (Cat. No. orb1524737) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Localization of GR Binding Sites by ChIP-sequencing. Chromatin from prednisolone treated THP-1 cells was immunoprecipitated with anti-GR antibody orb1524737 and analyzed by DNA sequencing. The figure illustrates the peak distribution of GR binding within a 250 Kb region of chromosome 6 as detected using anti-GR orb1524737.

Quick Database Links

Gene Symbol

GR

UniProt

P04150

RefSeq (Protein):NP_032199