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Catalog Number | orb1527172 |
---|---|
Category | Antibodies |
Description | FANCE Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 225 and 275 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | FANCE |
UniProt ID | Q9HB96 |
NCBI | NP_068741.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Fanconi anemia group E protein; Fanconi anemia com Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human FANCE by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FANCE antibody orb1527172 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. FANCE was also immunoprecipitated by rabbit anti-FANCE antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).