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Rabbit Estrogen Receptor Alpha Antibody
SKU: orb1530107
Description
Research Area
Cancer Biology, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, IHC, IP |
|---|---|
| Dilution Range | WB - Not recommended. Use rabbit anti-Estrogen Receptor Alpha antibody A300-498A.; IP - 3 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | Estrogen Receptor Alpha |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
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Detection of human Estrogen Receptor Alpha by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha used at a dilution of 1:2, 500 (0.4µg/ml).
Detection of human Estrogen Receptor Alpha by immunoprecipitation.Samples: Whole cell lysate from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ER alpha antibodies.
Localization of Estrogen Receptor Alpha Binding Sites by ChIP-sequencing. Chromatin from treated MCF-7 cells was immunoprecipitated with anti-Estrogen Receptor Alpha antibody and analyzed by DNA sequencing.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000116.2 |
|---|
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Datasheet
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Protocol Information
IHC
Immunohistochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
Rabbit Estrogen Receptor Alpha Antibody (orb1530107)
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