ESRRA Antibody (Center)

SKU: orb39322

Description

Peptide affinity purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22490
• Immunogen: Synthetic Peptide
• Target: ESRRA
• Molecular Weight: 45510

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Anti-Steroid hormone receptor ERR1 antibody, anti-Estrogen-related receptor antibody, anti-alpha antibody, anti-ERR-alpha antibody, anti-Estrogen receptor-like 1 antibody, anti-Nuclear receptor subfamily 3 group B member 1 antibody, anti-NR3B1 antibody, anti-ESRL1 antibody, anti-ERR1 antibody, anti-ESRRA antibody

Western blot analysis of ESRRA Antibody (Center) in A375 cell line lysates (35 ug/lane). ESRRA (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of ESRRA Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.

Flow cytometric analysis of 293 cells using ESRRA Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

ESRRA

UniProt

P11474

RefSeq (Protein):NP_001269379.1, NP_004442.3, NP_001269380.1