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Catalog Number | orb1527873 |
---|---|
Category | Antibodies |
Description | eIF2beta/EIF2S2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse |
Immunogen | Between 100 and 150 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-eIF2beta/EIF2S2 antibody A301-743A. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | eIF2beta/EIF2S2 |
UniProt ID | P20042 |
NCBI | NP_003899.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Eukaryotic translation initiation factor 2 subunit Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse eIF2beta/EIF2S2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2beta/EIF2S2 antibody orb1527873 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated more efficiently by rabbit anti-eIF2beta/EIF2S2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |