Rabbit eIF2beta/EIF2S2 Antibody

SKU: orb1527871

Description

eIF2beta/EIF2S2 Antibody

Research Area

Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 283 and C-terminus
• Target: eIF2beta/EIF2S2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF2; EIF2B; EIF2beta; eIF-2-beta; eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; PPP1R67; protein phosphatase 1, regulatory subunit 67

Detection of human and mouse eIF2beta/EIF2S2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2beta/EIF2S2 antibody orb1527871 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated less efficiently by rabbit anti-eIF2beta/EIF2S2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human eIF2beta/EIF2S2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2beta/EIF2S2 (Cat. No. orb1527871) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF2beta/EIF2S2

UniProt

P20042

RefSeq (Protein):NP_003899.2