Rabbit anti-EGFR Antibody

• Catalog Number: orb1530274
• Category: Antibodies
• Description: EGFR Antibody
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 1100 and 1150
• UniProt ID: P00533
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:10,000 - 1:50,000; IP - 1 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: erb-b2 receptor tyrosine kinase 1; proto-oncogene
• Note: For research use only
• NCBI: NP_005219.2
• Expiration Date: 12 months from date of receipt.

Detection of human EGFR by western blot.Samples: Whole cell lysate from HeLa and MCF-7 cells. Antibody: Affinity purified rabbit anti-EGFR antibody orb1530274 at 0.02 µg/ml.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1, 000 (1µg/ml).