Rabbit EGFR Antibody

SKU: orb1530272

Description

EGFR Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Monkey
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1160 and C-terminus
• Target: EGFR
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Detection of human EGFR by western blot.Samples: Whole cell lysate from HeLa cells. Antibody: Affinity purified rabbit anti-EGFR antibody orb1530272 at 0.04 µg/ml.

Detection of human and mouse EGFR by western blot.Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-EGFR antibody orb1530272 at 0.04 µg/ml.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1, 000 (1µg/ml).

Detection of human EGFR by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-EGFR antibody.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq (Protein):NP_958440.1