Rabbit EEA1 Antibody

SKU: orb1527594

Description

EEA1 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 100 and 150
• Target: EEA1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; MST105; MSTP105; ZFYVE2; zinc finger FYVE domain-containing protein 2

Detection of human EEA1 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-EEA1 antibody orb1527594 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human EEA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EEA1 antibody orb1527594 used for IP at 3 µg/mg lysate.

Detection of human EEA1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EEA1 (Cat. No. orb1527594) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

EEA1

UniProt

Q15075

RefSeq (Protein):NP_003557.2