EEA1 Rabbit Polyclonal Antibody

SKU: orb1294269

Description

Rabbit polyclonal antibody to EEA1

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:2000-1:4000, Immunofluorescence 1:500-1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: EEA1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Anti-EEA1 antibody - at 1/2000 dilution, Lysates at 35 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294269 overnight at 4°C.

EEA1 antibody by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons, Green: EEA1 antibody (orb1294269) diluted at 1:500. Red: Tuj1 antibody, Blue: DAPI.

EEA1 antibody by immunofluorescent analysis. Sample: MCF-7 cell, Red: EEA1 antibody (orb1294269) diluted at 1:500. Blue: DAPI.

Immunohistochemical analysis of paraffin embedded Human testis, breast, Colorental and lymph node cancer tissue labeling EEA1 with orb1294269 at 1/100.

Quick Database Links

Gene Symbol

EEA1