Rabbit anti-DDX3 Antibody

• Catalog Number: orb1530142
• Category: Antibodies
• Description: DDX3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DDX3
• UniProt ID: O00571
• NCBI: NP_001347.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: DEAD box protein 3, X-chromosomal; DEAD box, X iso
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DDX3 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-DDX3 antibody at 0.04 µg/ml.

Detection of human DDX3 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, MCF-7, Hep-G2, and Jurkat cells. Antibody: Affinity purified rabbit anti-DDX3 antibody orb1530142 at 0.04 µg/ml.

Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1µg/ml).

Detection of human DDX3 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-DDX3 antibody.