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Item 1 of 3
DDX3 Antibody (C-term)
Catalog Number: orb1935439
| Catalog Number | orb1935439 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB13438 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Mouse, Other |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 73243 Da |
| Target | This DDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-485 amino acids from the C-terminal region of human DDX3. |
| UniProt ID | O00571 |
| NCBI | NP_001180346.1, NP_001347.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | ATP-dependent RNA helicase DDX3X, DEAD box protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of DDX3 Antibody (C-term) in Hela cell line lysates (35 ug/lane). DDX3 (arrow) was detected using the purified Pab.
Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
