Rabbit anti-Cyclin T1 Antibody

SKU: orb1524725

Description

Cyclin T1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 325 and 375
• Target: Cyclin T1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

CYCT1; cyclin-T1; HIVE1; MLLT10/CCNT1 fusion; cyclin C-related protein; CDK9-associated C-type protein; CCNT; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1; Cyclin-T

Detection of human Cyclin T1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-Cyclin T1 antibody orb1524725 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Cyclin T1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cyclin T1 antibody orb1524725 used for IP at 6 µg/mg lysate.

Detection of human Cyclin T1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin T1 (Cat. No. orb1524725) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Cyclin T1

UniProt

O60563

RefSeq (Protein):NP_001231.2