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Item 1 of 4
Item 1 of 4
CCNT1 Antibody (Center)
Catalog Number: orb1938808
| Catalog Number | orb1938808 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19562 |
| Tested applications | FC, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 80685 Da |
| Target | This CCNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human CCNT1. |
| UniProt ID | O60563 |
| NCBI | NP_001231.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cyclin-T1, CycT1, Cyclin-T, CCNT1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-CCNT1 Antibody (Center) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCNT1 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).
CCNT1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35 ug/lane). This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).
