Rabbit CDKL5 Antibody

SKU: orb1523486

Description

CDKL5 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 350 and 400
• Target: CDKL5
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CFAP247; cyclin dependent kinase 5 transcript; cyclin-dependent kinase-like 5; EIEE2; ISSX; serine/threonine kinase 9; serine/threonine-protein kinase 9; STK9

Detection of human CDKL5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody orb1523486 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human CDKL5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody orb1523486 used for IP at 6 µg/mg lysate.

Detection of human CDKL5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDKL5 (Cat. No. orb1523486) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human CDKL5 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDKL5 (Cat. No. orb1523486) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

CDKL5

UniProt

O76039

RefSeq (Protein):NP_003150.1