Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

SKU: orb1519887

Description

CD8 alpha Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR044F
• Immunogen: Between 185 and C-terminus
• Target: CD8 alpha
• Purity: Recombinant antibody was purified from cell culture supernatant

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 250 µg/ml
• Disclaimer: For research use only

Alternative Names

Leu2 T-lymphocyte antigen; T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain; T-cell antigen Leu2; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; Leu2; CD8 antigen, alpha polypeptide (p32); CD8; CD antigen CD8a; p32

Detection of human CD8 alpha by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG). Substrate: DAB

Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human melanoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Detection of human CD8 alpha (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Detection of human CD8 alpha by western blot. Samples: Whole cell lysate (50 µg) from CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Recombinant monoclonal antibodies to CD3e and CD4

Quick Database Links

Gene Symbol

CD8 alpha

UniProt

P01732

RefSeq (Protein):NP_001759.3