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Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

Catalog Number: orb1519887

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1519887
CategoryAntibodies
DescriptionCD8 alpha Antibody
TargetCD8 alpha
ClonalityRecombinant
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceWhole IgG
Concentration250 µg/ml
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
PurityRecombinant antibody was purified from cell culture supernatant
ImmunogenBetween 185 and C-terminus
UniProt IDP01732
Tested applicationsFC, IF, IHC, WB
Dilution rangeFlow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Application notesFormat: Whole IgG
Antibody TypePrimary Antibody
Clone NumberBLR044F
Storage2 - 8°C
Alternative namesLeu2 T-lymphocyte antigen; T-lymphocyte differenti
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BackgroundThe CD8 antigen is a cell surface glycoprotein fou
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NoteFor research use only
NCBINP_001759.3
Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

Detection of human CD8 alpha by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG). Substrate: DAB

Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human melanoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

Detection of human CD8 alpha (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

Detection of human CD8 alpha by western blot. Samples: Whole cell lysate (50 µg) from CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Recombinant monoclonal antibodies to CD3e and CD4