CD8 Recombinant Rabbit Monoclonal Antibody

SKU: orb1610270

Description

CD8 Recombinant Rabbit Monoclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: D5A7
• Immunogen: KLH conjugated synthetic peptide derived from human CD8
• Target: CD8A
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD8; CD8alpha; IMD116; Leu2; p32; Ly-2; Ly-35; Ly-B; Lyt-2; CD8A_HUMAN; CD8A; T-lymphocyte differentiation antigen T8/Leu-2; MAL; CD8A_MOUSE; T-cell surface glycoprotein Lyt-2; Lyt2; G1SX34_RABIT;

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD8) Monoclonal Antibody, Unconjugated (orb1610270) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD8) Monoclonal Antibody, Unconjugated (orb1610270) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD8A

UniProt

P01732