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Catalog Number | orb1519975 |
---|---|
Category | Antibodies |
Description | CD45 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BL-178-12C7 |
Tested applications | FC, ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 1256 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 50 µg/ml |
Dilution range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CD45 |
UniProt ID | P08575 |
NCBI | NP_002829.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | L-CA; protein tyrosine phosphatase, receptor type, Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975) used for IP at 20 µl/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (orb1519975). For blotting immunoprecipitated CD45, orb1519975 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.
Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |