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Rabbit anti-CD4 Recombinant Monoclonal Antibody

Catalog Number: orb1519966

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1519966
CategoryAntibodies
DescriptionCD4 Antibody
TargetCD4
ClonalityRecombinant
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceWhole IgG
Concentration1000 µg/ml
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
PurityRecombinant antibody was purified from cell culture supernatant
ImmunogenResidues 26-397 (ECD)
UniProt IDP01730
Tested applicationsFC, IF, IHC, IP, WB
Dilution rangeFlow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 6 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Application notesAll western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
Antibody TypePrimary Antibody
Clone NumberBL-155-1C11
Storage2 - 8°C
Alternative namesCD4mut; T-cell surface antigen T4/Leu-3; T-cell su
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BackgroundCD4 is a membrane glycoprotein of T lymphocytes th
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NoteFor research use only
NCBINP_000607.1
Rabbit anti-CD4 Recombinant Monoclonal Antibody

Detection of human CD4 by western blot. Samples: Whole cell lysate (50 µg) from U-937, HEK293T, MOLT-4, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody [BL-155-1C11] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Rabbit anti-CD4 Recombinant Monoclonal Antibody

Detection of human CD4 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Rabbit anti-CD4 Recombinant Monoclonal Antibody

Detection of human CD4 (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG

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