Rabbit anti-CD4 Recombinant Monoclonal Antibody

SKU: orb1519574

Description

CD4 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:1,000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR167J
• Immunogen: Between 407 and C-terminus
• Target: CD4
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

L3T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; Ly-4; CD antigen CD4; T-cell differentiation antigen L3T4

Detection of mouse CD4 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, BW5147.3, thymus, 70Z/3, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD4 by immunohistochemistry. Sample: FFPE section of mouse thymus. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD4 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (200 µg per IP reaction; 20% of IP loaded) from mouse thymus. Antibodies: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574) used for IP at 6 µl/200 µg lysate. CD4 was also immunoprecipitated by a previous lot of this antibody (orb1519574). For blotting immunoprecipitated CD4, orb1519574 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Quick Database Links

Gene Symbol

CD4

UniProt

P06332

RefSeq (Protein):NP_038516.1