Rabbit cAbl Antibody

SKU: orb1528007

Description

cAbl Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 550 and 600
• Target: cAbl
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; ABL; bcr/abl; bcr/c-abl oncogene protein; c-ABL; c-abl oncogene 1, receptor tyrosine kinase; c-ABL1; CHDSKM; JTK7; p150; proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; v-abl; v-abl Abelson murine leukemia viral oncogene homolog 1

Detection of human cAbl by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-cAbl antibody orb1528007 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. cAbl was also immunoprecipitated by rabbit anti-cAbl antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human cAbl by immunohistochemistry and immunocytochemistry.Sample: FFPE section of human prostate carcinoma (left) and formaldehyde-fixed K-562 cells (contains the chromosomal translocation, t (9: 22) that creates the BCR/ABL fusion gene) (right). Antibody: Affinity purified rabbit anti-cAbl (Cat. No.orb1528007) used at a dilution of 1: 1, 000 (0.2µg/ml) and 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

cAbl

UniProt

P00519

RefSeq (Protein):NP_005148.2