Rabbit c-Kit Recombinant Monoclonal Antibody

SKU: orb1733238

Description

c-Kit Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR263L
• Immunogen: Between 325 and 375
• Target: c-Kit
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD antigen CD117; CD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Detection of human c-KIT by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEL 92.1.7, HEK293T, HUVEC-C, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733238) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human c-KIT by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733238). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human c-KIT by immunocytochemistry. Sample: FFPE section of human HEL 92.1.7 cells. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733238). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

c-Kit

UniProt

P10721

RefSeq (Protein):NP_000213.1