C-Kit Recombinant Rabbit Monoclonal Antibody

SKU: orb559218

Description

C-Kit Recombinant Rabbit Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:1000-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 7C8
• Immunogen: Recombinant human c-Kit protein, around C-terminal 200aa
• Target: KIT
• Molecular Weight: 105 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-Kit; CD117; MASTC; PBT; SCFR; Bs; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; SCO1; SCO5; SOW3; Ssm; Tr-kit; W; KIT_HUMAN; KIT; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; 2.7.10.1; KIT_MOUSE; Sl;

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

KIT

UniProt

P10721