Rabbit BRD2 Antibody

SKU: orb1526370

Description

BRD2 Antibody

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; ICC-IF - 1:50 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: BRD2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3

Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody orb1526370 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody orb1526370 used for IP at 6 µg per reaction.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody orb1526370 used for IP at 6 µg per reaction.

Detection of human BRD2 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.orb1526370) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

BRD2

UniProt

P25440

RefSeq (Protein):NP_005095.1