Rabbit BRD2 Antibody

SKU: orb1526368

Description

BRD2 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 700 and 750
• Target: BRD2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3

Detection of mouse BRD2 by western blot. Samples: Whole cell lysate (50 µg) from RenCa and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by a previous lot of this antibody (orb1526368) and a second antibody against a different epitope of BRD2.

Detection of human BRD2 by immunohistochemistry.Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (orb1526368) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human BRD2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Quick Database Links

Gene Symbol

BRD2

UniProt

P25440

RefSeq (Protein):NP_005095.1