Rabbit Beta-Arrestin 2 Antibody

SKU: orb1524183

Description

Beta-Arrestin 2 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 359 and C-terminus
• Target: Beta-Arrestin 2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARB2; ARR2; arrestin 3; Arrestin beta-2; BARR2; beta-arrestin-2; non-visual arrestin-3

Detection of human Beta-Arrestin 2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Beta-Arrestin 2 antibody orb1524183 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Beta-Arrestin 2 was also immunoprecipitated by rabbit anti-Beta-Arrestin 2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human Beta-Arrestin 2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Beta-Arrestin 2 Cat. No. orb1524185 used at a dilution of 1: 1, 000 (1µg/ml) (left) and Cat. No. orb1524183 used at a dilution of 1: 1, 000 (1µg/ml) (right). Detection: DAB

Detection of human Beta-Arrestin 2 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-Beta-Arrestin 2 Cat. No. orb1524185 used at a dilution of 1: 1, 000 (1µg/ml) (left) and Cat. No. orb1524183 used at a dilution of 1: 1, 000 (1µg/ml) (right). Detection: DAB

Quick Database Links

Gene Symbol

Beta-Arrestin 2

UniProt

P32121

RefSeq (Protein):NP_945355.1