Rabbit anti-BCR Antibody

• Catalog Number: orb1527312
• Category: Antibodies
• Description: BCR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IP, WB
• Reactivity: Human
• Immunogen: Between 1221 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 10 µg/mg lysate; ICC-IF - 1:500 - 1:2,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BCR
• UniProt ID: P11274
• NCBI: NP_004318.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: D22S11; PHL; BCR/FGFR1 chimera protein; FGFR1/BCR
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BCR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BCR antibody orb1527312 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human BCR by immunocytochemistry.Sample: FFPE section of human K-562 cells (contains the chromosomal translocation, t (9: 22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR (Cat. No. orb1527312) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Green-fluorescent goat anti-rabbit IgG-Alexa Fluor® 488 used at a dilution of 1: 1, 000.