Rabbit anti-ASCT2 Antibody

• Catalog Number: orb1523154
• Category: Antibodies
• Description: ASCT2 Antibody
• Target: ASCT2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 491 and C-terminus
• UniProt ID: Q15758
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: R16; ASCT2; Solute carrier family 1 member 5; solu
• Note: For research use only
• NCBI: NP_005619.1

Detection of human ASCT2 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASCT2 antibody (orb1523154) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ASCT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASCT2 antibody (orb1523154) used for IP at 6 µg per reaction. ASCT2 was also immunoprecipitated by a previous lot of this antibody (orb1523154) and a second antibody against a different epitope of ASCT2.