Rabbit API5 Antibody

SKU: orb1525998

Description

API5 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Xenopus
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: API5
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

AAC11; AAC-11; antiapoptosis clone 11 protein; API-5; apoptosis inhibitor 5; cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; fibroblast growth factor 2-interacting factor 2; FIF; migration-inducing protein MIG8; Protein XAGL

Detection of human and mouse API5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody orb1525998 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody orb1525998 used for IP at 3 µg per reaction.

Detection of human API5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525998) used at 1: 200 (1 µg/ml). Detection: DAB

Detection of mouse API5 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525998) used at 1: 200 (1 µg/ml) Detection: DAB

Quick Database Links

Gene Symbol

API5

UniProt

Q9BZZ5

RefSeq:XP_508375.2