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API5 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50 - 1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | apoptosis inhibitor 5 |
| Target | API5 |
| Molecular Weight | 50-55 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
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Immunofluorescent analysis of 10% Formaldehyde fixed HepG2 cells using orb624774 (API5 antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Rabbit IgG (H+L).

HepG2 cells were subjected to SDS PAGE followed by western blot with orb624774 (API5 Antibody) at dilution of 1:300.

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using orb624774 (API5 Antibody) at dilution of 1:50.
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API5 Antibody (orb624774)
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