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Rabbit AMPK alpha 1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 375 and 425 |
| Target | AMPK alpha 1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human AMPK alpha1 by western blot.Samples: Whole cell lysate from A-549, Jurkat, U2OS, HEK293T, and HeLa cells. Antibody: Affinity purified rabbit anti-AMPK alpha1 antibody at 0.04 µg/ml.

Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human AMPK alpha1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-AMPK alpha1 antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_006242.5 |
|---|
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Protocol Information
Rabbit AMPK alpha 1 Antibody (orb1530091)
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