Rabbit anti-alpha-Tubulin Antibody

SKU: orb1520421

Description

alpha-Tubulin Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2-10 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 175 and 225
• Target: alpha-Tubulin

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

tubulin alpha-1A chain; hum-a-tub1; tubulin, alpha, brain-specific; tubulin B-alpha-1; tubulin alpha-3 chain; Alpha-tubulin 3; hum-a-tub2; B-ALPHA-1; TUBA3; LIS3

Detection of mouse TUBA1A by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TUBA1A antibody (orb1520421) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TUBA1A by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TUBA1A antibody (orb1520421) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TUBA1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TUBA1A antibody (orb1520421) used for IP at 1 µg per reaction.

Quick Database Links

Gene Symbol

alpha-Tubulin

UniProt

Q71U36

RefSeq (Protein):NP_001257328.1