Tubulin-alpha Rabbit Polyclonal Antibody (Loading Control)

• Catalog Number: orb11535
• Category: Antibodies
• Description: Tubulin-alpha Rabbit Polyclonal Antibody (Loading Control)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Tubulin-alpha 1 (375-448/448aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBA1A
• UniProt ID: Q71U36
• RRID: AB_10746293
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha tubulin 1; alpha-tubulin 1; Detyrosinated al
• Background: _CLIENT_OVERRIDE_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 Cells (blue). Primary Antibody: Rabbit Anti-Tubulin-alpha 1 antibody (orb11535), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (orb11535, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human Jurkat cell lysates, Lane 3: Human SH-SY5Y cell lysates, Lane 4: Human U-2 OS cell lysates, Lane 5: Human A431 cell lysates, Primary: Anti-Tubulin-alpha (orb11535) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 52 kDa.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human 293T cell lysates, Primary: Anti-Tubulin-alpha (orb11535) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 52 kDa.