TUBA1C Rabbit Polyclonal Antibody

SKU: orb7128

Description

TUBA1C Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Tubulin alpha 6 (51-150/449aa)
• Target: TUBA1C
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

alpha tubulin 6; alpha tubulin isotype M alpha 6; bcm948; M[a]6; TUBA1C; Tubulin alpha 1C chain; Tubulin alpha-1C chain; tubulin alpha 6; Tubulin alpha 6 chain; tubulin, alpha 1c; TBA1C_HUMAN; alpha-tubulin 6; Tubulin alpha-6 chain.

Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TUBA1C antibody (orb7128), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TUBA1C) Polyclonal Antibody, Unconjugated (orb7128) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Rat Placenta tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Human K562 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human Jurkat cell lysates, Primary: Anti-TUBA1C (orb7128) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

TUBA1C

UniProt

Q9BQE3