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Catalog Number | orb556641 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to alpha Tubulin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC-P, WB |
Reactivity | Drosophila, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Concentration | 0.25 mg/ml |
Dilution range | WB: 1:500-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
Form/Appearance | Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Purity | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
MW | 50 |
Target | alpha Tubulin |
UniProt ID | Q71U36 |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
Buffer/Preservatives | 0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol |
Alternative names | tubulin alpha 1a , B-ALPHA-1 , LIS3 , TUBA3 Read more... |
Note | For research use only |
Application notes | Positive control: NIH-3T3 , mouse brain , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , rat brain |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of HeLa cells using alpha Tubulin antibody
Immunofluorescence analysis of HeLa cells using alpha Tubulin antibody
Immunohistochemical staining of SAS xenograft using alpha Tubulin antibody
Western blot analysis of Various whole cell using alpha Tubulin antibody
Western blot analysis of Rat brain using alpha Tubulin antibody
Western blot analysis of NIH-3T3 whole cell(Lane 1), mouse brain(Lane 2) using alpha Tubulin antibody
Western blot analysis of NIH-3T3 whole cell(Lane 1), mouse brain(Lane 2) using alpha Tubulin antibody
Western blot analysis of drosophila lysate using alpha Tubulin antibody
IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, IP, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |