Rabbit anti-AF6 Antibody

SKU: orb1527034

Description

AF6 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1500 and 1550
• Target: AF6

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

l-afadin; protein AF-6; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; MLL-AF6; ALL1-fused gene from chromosome 6 protein; afadin; AF6; adherens junction formation factor; MLLT4

Detection of human and mouse AF6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF6 antibody orb1527034 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human AF6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AF6 antibody orb1527034 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

AF6

UniProt

P55196

RefSeq:BAA32484.1