Rabbit ABC50 Antibody

SKU: orb1527409

Description

ABC50 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-ABC50 antibody A301-999A.; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Guinea pig, Monkey, Porcine, Rabbit
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 795 and C-terminus
• Target: ABC50
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ABC27; ABC50; ATP-binding cassette 50; ATP-binding cassette 50 (TNF-alpha stimulated); ATP-binding cassette sub-family F member 1; ATP-binding cassette, sub-family F (GCN20), member 1; TNFalpha-inducible ATP-binding protein; TNF-alpha-stimulated ABC protein

Detection of human ABC50 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ABC50 antibody orb1527409 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. ABC50 was successfully immunoprecipitated by rabbit anti-ABC50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

Detection of human ABC50 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABC50 (Cat. No. orb1527409) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ABC50

UniProt

Q8NE71

RefSeq (Protein):NP_001081.1