PUMA rabbit pAb

SKU: orb766160

Description

Rabbit polyclonal antibody to PUMA

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169
• Molecular Weight: 23
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-BBC3 antibody, anti-PUMA antibody, anti-Bcl-2-binding component 3 antibody, anti-JFY-1 antibody, anti-p53 up-regulated modulator of apoptosis antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of various cells using PUMA Polyclonal Antibody.

Western blot analysis of lysate from 293 cells treated with EGF, using PUMA antibody.