PUM2 Antibody

• Catalog Number: orb1247050
• Category: Antibodies
• Description: PUM2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DQNRDLKQGDDDDSK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration 0.3-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PUM2
• UniProt ID: Q8TB72
• NCBI: NP_056132.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PUM2, PUMH2, PUML2, KIAA0235, pumilio homolog 2 (D
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration 0.3-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed with orb1247050 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1247050 (1 mg/ml) in Lane B.

orb1247050 (10 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.